Risk of adverse pregnancy and infant outcomes associated with prenatal Zika virus infection: a post-epidemic cohort in Central-West Brazil

This study aimed to estimate the risks of adverse infant outcomes in the first year of life related to prenatal Zika virus (ZIKV) exposure. A prospective cohort of pregnant women with rash was recruited in Central-West Brazil in a post-epidemic period (January 2017 to April 2019). We evaluated participants’ medical histories and performed ZIKV diagnostic testing using molecular (reverse transcription polymerase chain reaction [RT-PCR]) and serologic (immunoglobulin [Ig]M and plaque reduction neutralization tests [PRNT90]) assays. The ZIKV-positive group included both RT-PCR-confirmed cases as well as IgM and/or PRNT90-positive probable cases. Children were evaluated at birth and in the first 12 months of life. Transfontanellar ultrasound, central nervous system computed tomography, eye fundoscopy and retinography were performed. We estimated the absolute risk and 95% confidence interval (95% CI) of adverse infant outcomes among confirmed prenatally ZIKV-exposed children. Among 81 pregnant women with rash, 43 (53.1%) were ZIKV infected. The absolute risk of microcephaly among offspring of ZIKV-infected pregnant women was 7.0% (95% CI: 1.5–19.1), including the two cases of microcephaly detected prenatally and one detected postnatally. In total, 54.5% (95% CI: 39.8–68.7) of children in the ZIKV-exposed group had at least one ophthalmic abnormality, with the most frequent abnormalities being focal pigmentary mottling and chorioretinal atrophy or scarring. Our findings reinforce the importance of long-term monitoring of prenatally ZIKV-exposed children born apparently asymptomatic for Congenital Zika Syndrome.


Results
Between January 2017 and April 2019, 125 pregnant women with rash and suspected ZIKV infection were identified in the city of Goiânia, Goiás, Brazil. Of the 81 who agreed to participate and were included in this cohort study, 43 (53.1%) women had laboratory-confirmed ZIKV infection based on molecular and/or serological tests. Of these 43 cases, 34 (79.1%) were confirmed positive for ZIKV maternal infection by RT-PCR, 5 (11.6%) by IgM and PRNT 90 , and four (9.3%) by IgM alone. Among the ZIKV-infected pregnant women, we detected one coinfection with chikungunya virus (CHIKV by RT-PCR) and one with dengue virus (DENV by PRNT 90 ).
Thirty-eight pregnant women tested negative for ZIKV, of whom 14 (36.8%) were DENV-positive by molecular or serological tests (Fig. 1). Table 1 shows the sociodemographic characteristics of the enrolled pregnant women and the frequency of symptoms associated with the acute rash disease, according to laboratory results. The comparison was made between the group of ZIKV-positive pregnant women (n=43) versus the ZIKV-negative group (n=38). Most participants (n=79, 97.6%) were recruited during 2017-2018. In the ZIKV-positive group, 67.4% (n=29) of the pregnant women were recruited in 2017, and in contrast 89.4% (n=34) of the negative group were recruited in 2018. Only two cases were recruited in 2019 (up to April), and they were both ZIKV-negative. Among ZIKVpositive pregnant women, participants' ages ranged from 16 to 39 years old (mean of 26.4), 75% reported having more than nine years of schooling, 63% had a family income higher than two times the Brazilian minimum wage per month, and 61% were of parda (brown). Overall, the frequency of acute exanthematic disease was distributed across pregnancy trimesters, with approximately one-third of cases in each trimester of pregnancy. The ZIKVrelated rashes lasted for a median duration of four days and were accompanied mainly by pruritus, headache, prostration, arthralgia, myalgia, and fever. Other symptoms, including retro-orbital pain, photophobia, and upper respiratory symptoms, were reported less frequently. Conjunctival hyperemia was more common in the ZIKV-positive group (53.1%, n=10) versus the ZIKV-negative group (7.9%, n=3).
We followed up 71 pregnant women and 74 children (i.e., from 68 singleton and three twin pregnancies). The three pairs of twins were in the maternal ZIKV-positive group. In total, 95.8% (n=68) of participants had serological screening data for syphilis, toxoplasmosis, cytomegalovirus (CMV), rubella, and HIV. All were HIV-negative and had no evidence of syphilis, rubella nor recent CMV infection. Two pregnant women had IgM-positive test results for toxoplasmosis, and both were in the ZIKV-negative group. Table 2 shows the characteristics of the prenatal follow-up until delivery. All pregnant women had at least two obstetric ultrasounds, and 76% (n=54) had three ultrasounds (one per trimester). Six cases of fetal abnormalities were detected by obstetric ultrasound.
In this pregnancy cohort, there were no miscarriages or stillbirths. During the gestational period, 13.1% of women required hospitalization. Overall, the most frequent comorbidities during pregnancy were hypertension (20.0%) with one eclampsia case, urinary tract infection (17.5%) and gestational diabetes (12.5%), with no statistical difference between groups. Premature delivery occurred in 14.3% of the ZIKV-positive group versus 3.7% of the ZIKV-negative group. There were similar frequencies of labor induction and elective delivery between the ZIKV-positive and ZIKV-negative groups. Cesarean section (61.8%) was the most common delivery route in both groups (Table 2).
Overall, 72 newborns, of whom 45 were children in the maternal ZIKV-positive group and 27 were in the ZIKV-negative group, were evaluated from delivery up to one year of age ( www.nature.com/scientificreports/ one from each group, were lost to follow-up just before labor. The median values for APGAR scores in the 5th minute, weight, length at birth and head circumferences were similar between the groups. Four neonates with microcephaly were detected at birth, all in the ZIKV-exposed group. In addition, morphological malformations in newborns and children were investigated by clinical evaluation (pediatrician, ophthalmologist, and neurologist) and complementary exams. In total, the mean number of clinical evaluations per child was 3.1 (standard deviation, SD: 1.1) appointments. Children with maternal ZIKV exposure had significantly greater chances of follow-up than unexposed children in the first year of life (Table 3).
All children with microcephaly and/or brain imaging abnormalities were in the ZIKV-exposed group. Four infants had microcephaly detected at birth. Of these, two microcephalic infants had ZIKV exposure in the 1st trimester, prenatal morphological alterations confirmed by transfontanellar ultrasound (i.e., unilateral ventriculomegaly, periventricular calcifications, and cortical atrophy), and were classified as having postpartum disproportional microcephaly. Additionally, one of the two children with disproportional microcephaly was classified as having severe microcephaly, occipital bulge, calcification of the cranial sutures, and arthrogryposis. Both children with disproportional microcephaly had severely impaired neurodevelopment during medical follow-up and fulfilled the criteria for severe complications compatible with CZS. The other two cases, classified as having mild proportional microcephaly at birth, had normal postpartum growth and development over the first year of life. Both fetuses had intrauterine growth restriction and normal neuroimaging results at prenatal ultrasound. One child with proportional microcephaly presented with chorioretinal atrophy and focal pigmentary mottling and was classified as having mild/moderate signs potentially associated with CZS. The other child with proportional microcephaly presented with chorioretinal atrophy as the only abnormality detected. Supplementary Table (S1) presents the main clinical characteristics, prenatal and postnatal imaging, and ZIKV RT-PCR results for all six children with brain abnormalities potentially associated with CZS.
Among the ZIKV-exposed group, two children, born with normal head circumferences, presented with neurological abnormalities by transfontanellar ultrasound. One child, with linear perithalamic calcifications, maintained a normal head circumference but presented with neurological delays during follow-up and was classified as having mild/moderate signs potentially associated with CZS, without microcephaly. The other child had frontoparietal calcifications, bilateral ventriculomegaly, and developed severe postnatal microcephaly, abnormal neurological development, and was classified as having severe complications compatible with ZIKV. Of note, two children in the unexposed group also had mild abnormalities at the neurological assessment at three months of age; one had abnormal tone/trophism, while the other had inadequate visual response and mild motor deficit. Both had normal development upon subsequent clinical evaluations.
Overall, 10 out of 61 (16.4%) children had minor heart abnormalities detected on transthoracic echocardiogram (ECHO) during the neonatal period. Of these, seven cases had patent foramen ovale (PFO), two had ostium secundum septal defect, and one had tricuspid physiological regurgitation. Four of the children with heart abnormalities were premature, with gestational ages ranging from 30.4 to 36.6 (weeks.days). Minor heart defects were detected in 21.6% (8/37) of the ZIKV-exposed group and in 8.3% (2/24) of the unexposed group. Of note, one child with microcephaly had echogenic intracardiac structure (golf ball) at prenatal ultrasound and also 3 mm PFO on ECHO. Overall, no severe cardiac defects were detected. Table 4 presents the main adverse outcomes among the ZIKV-exposed fetuses and children. Considering the obstetrical ultrasound findings 5.0% (95% CI: 0.9-15.5) of fetuses presented with microcephaly, cerebral www.nature.com/scientificreports/ calcifications and ventriculomegaly prenatally. The absolute risk of microcephaly at birth was 9.1% (95% CI: 3.7-17.9). The risk of microcephaly within one year of age was 7.0% (95% CI: 1.5-19.1) including two cases detected prenatally and one detected postnatally. These three children with microcephaly met the criteria for severe complications compatible with CZS. Two children without microcephaly presented with signs potentially associated with mild/moderate CZS. Therefore, the absolute risk of mild/moderate to severe CZS was 11.4% (95% CI: 4.3-23.4). In total, 54.5% (95% CI: 39.8-68.7) of ZIKV-exposed children presented at least one ophthalmological anomalies (retinal and/or optic nerve).

Discussion
Approximately two years after the peak of the ZIKV epidemic in Brazil, we detected three microcephaly cases (7.0%) among a cohort of ZIKV-positive pregnant women in the Central-West region of Brazil. In our study, most participants were recruited in 2017 and 2018. Whereas 67% of the ZIKV-positive women were detected in 2017, 89% of the ZIKV-negative group were recruited in 2018. Consistent with previous findings from an ecological study using surveillance data from Goiânia 18 , our findings underscore the persistence of the ZIKV circulation beyond the peak in 2015-2016 but also highlight the rapid decline of ZIKV transmission in subsequent years. These observations are also consistent with national surveillance data 19,20 as well as the broader epidemiological trends in ZIKV circulation across the American region 21 .
In our cohort of 81 pregnant women with rash, 43 were laboratory confirmed to have ZIKV. In addition, DENV was detected in 50% of the ZIKV-negative group, showing the cocirculation of ZIKV and DENV in our study setting. These findings are consistent with the Brazilian surveillance system and other field studies conducted in urban areas with high populations of Aedes spp. mosquitoes 22,23 . At baseline, pregnant women with rash had similar clinical presentations regardless of whether they were positive for ZIKV, DENV, or CHIKV, as described in other Brazilian regions [24][25][26][27] . Due to the similarity of clinical manifestations, short viremia duration, and the serological cross-reactivity between flaviviruses (i.e., DENV and ZIKV), it is urgent to have highly specific point of care molecular tests for rapidly differentiating between DENV, ZIKV, and CHIKV in endemic regions. We also identified two ZIKV infected pregnant women simultaneously infected with CHIKV or DENV, but they did not present with more severe clinical presentations. These findings align with a recent systematic review that provided evidence that ZIKV coinfections, mainly with CHIKV or DENV, do not appear to influence the clinical manifestation of uncomplicated ZIKV infections in adults in general 28 .
In the present study, prenatal ultrasound exams detected two fetuses with microcephaly, cerebral calcifications, and ventriculomegaly diagnosed as CZS. Abnormal prenatal ultrasonography results were well documented in a cohort of 92 ZIKV-infected women during the emergence of ZIKV in Rio de Janeiro, Brazil 3 . However, normal ultrasound results do not exclude the risk of adverse outcomes at birth 29 . In Brazil, reports of CZS were mainly Table 2. Characteristics of prenatal follow-up until delivery of women with rash and suspected ZIKV infection, Goiânia, Goiás, Brazil, 2017-2019. a Abnormalities might be in both trimesters. b One or more complications are possible at the same woman and/or fetus. Numbers may vary due to missing values.

Variables
Total N=71 (%)  www.nature.com/scientificreports/ diagnosed in the postnatal period, and only 2% of the cases were identified by prenatal ultrasound 30 , suggesting potential underreporting of congenital anomalies in the official surveillance system. Our result of an 11.4% risk of CZS is consistent with the 12.7% risk of severe adverse and neonatal outcomes reported in a cohort of pregnant women residing in French Guiana that was conducted in the epidemic and postepidemic period 31 . However, our finding was higher than the 3.9% (95% CI: 2.5-5.9) risk of CZS, microcephaly and/or other central nervous system malformations reported among live births during the peak of the epidemic in Southeast of Brazil 32 . These discrepancies are likely explained, in part, by differences in the length of follow-up as well as the performance of in-depth eye examinations (e.g., retinography).
In our cohort, the estimated absolute risk of microcephaly among children born to ZIKV-infected pregnant women was 7.0% (95% CI: 1.5-19.1), including the two cases detected prenatally and one case with microcephaly detected postnatally. Of note, all three children with Zika-related microcephaly were born to pregnant women   www.nature.com/scientificreports/ with rash reported in the first trimester of the gestational period. This finding is in line with other studies that have estimated the risk of microcephaly and/or other major signs of CZS among offspring of pregnant women with ZIKV infection to vary from 1.9 to 4.5% 3,24,29,32,33 . One question raised in the early ZIKV pandemic was whether the absolute risk of Zika-related microcephaly varied according to the geographic setting 34 . A recent meta-analysis of 13 Brazilian cohorts of ZIKV infected women showed strong evidence that the risk of microcephaly was relatively homogeneous across the regions 12 . Therefore, the variation of risk among the different cohort studies may be due to study design-related discrepancies, such as the different criteria of maternal exposure and the range of short and long-term outcomes evaluated 35 .
Regarding the broad spectrum of congenital ZIKV infection, one of the key findings from our research is the high prevalence (~55%) of eye defects detected in ZIKV-exposed children with or without brain compromise. This high frequency of ocular abnormalities may be due to the early fundoscopic eye exam that identified patients to undergo retinography. The retinography offers a good posterior pole detail, allowing the early detection of subtle anomalies such as focal pigmentary mottling.
In a cohort conducted in Rio de Janeiro, 21.4% of prenatally ZIKV-exposed infants had eye abnormalities 36 , primarily in the absence of other neurological abnormalities. Other studies have reported a high frequency of eye abnormalities ranging from 21.4% to 70.0% among children with CZS, with optic nerve and retinal abnormalities as the most common findings 37,38 . This large variation may be due to differences in participants` inclusion criteria, diagnostic tools, and follow-up time. Although uncertainty remains regarding the clinical significance of minor eye anomalies in infants without other manifestations of CZS, prolonged monitoring of these children and their vision remains important.
In our cohort, approximately one-fourth of the ZIKV-exposed group had minor cardiac findings, mainly persistent foramen ovale, detected on ECHO during the neonatal period. Persistent foramen ovale is a common asymptomatic defect expected to resolve spontaneously with increasing age. Other studies have also reported high frequencies of minor/mild heart defects noted on ECHO among ZIKV-exposed infants 39-41 that are higher among ZIKV-exposed infants than expected for the population 42 . This higher prevalence of heart defects among prenatally ZIKV-exposed children may be explained by the use of a sensitive screening method (ECHO) to investigate heart defects in asymptomatic neonates. So far, there is no evidence of the association between ZIKV infection and congenital heart defects. Currently, heart defects are not considered adverse outcomes of congenital ZIKV infection in position papers and guidelines 16,[43][44][45] .
The strengths of our study consist of its prospective design using a standardised protocol in collaboration with the Microcephaly Epidemic Research Group (MERG, Brazil) as part of the ZikaPLAN consortium 46,47 . In addition, laboratory evidence of recent maternal ZIKV exposure by molecular and serological tests were performed in a reference laboratory. A team of specialists investigated fetal and postnatal adverse outcomes up to the first year of life. One limitation of the study is that not all neonates/infants were tested for ZIKV infection due to difficulty in collecting specimens from apparently healthy infants without clinical signs/symptoms. Another limitation is that the children prenatally exposed to ZIKV had higher adherence to scheduled medical visits compared to the ZIKV-negative group. While a possible follow-up bias cannot be excluded, it does not affect the estimated absolute risks among the ZIKV-exposed group. This cohort is also limited by its small sample size. Nevertheless, our study represents approximately 90% of the pregnant women with rash and confirmed ZIKV infection notified to the local surveillance system in the study period. The small number of pregnant women with rash registered in the post-outbreak period in Goiânia hampered our ability to estimate less frequent adverse pregnancy outcomes, such as stillbirth. Also, this type of passive surveillance that excludes the asymptomatic cases clearly underestimates the entire ZIKV-positive women population in the local community. Implementation of prenatal ZIKV screening during non-epidemic years remains under discussion 48 .
Our study highlighted the relevance of prenatal ultrasound findings in the early diagnosis of CZS. Further, our research underscores the urgent need for point of care molecular tests to distinguish arbovirus infections due to the overlap of clinical signs, the short viremia period, and the serological cross-reactivity among flaviviruses. Our analysis, particularly our ophthalmological findings, also reinforces the importance of long-term follow-up of prenatally ZIKV-exposed children born apparently asymptomatic for CZS. Finally, congenital ZIKV infection continues to affect neonates in the post-epidemic period, and it is a persistent threat in endemic areas, such as Brazil. Vaccines to prevent maternal ZIKV infections are urgently needed in regions with Aedes spp. mosquitoes.  50 . In early 2017, we established a prenatal clinic for exanthematic diseases at the public referral hospital for high-risk pregnancies and pediatric care (Hospital Estadual Materno Infantil Dr. Jurandir do Nascimento, HMI), where this cohort was based.

Methods
Potentially eligible participants for this study were identified (i) from records in the compulsory SINAN notification system for ZIKV infections during pregnancy or (ii) from those seeking attendance at the emergency www.nature.com/scientificreports/ ward and referred to the prenatal clinic for exanthematic diseases at HMI and, then, were invited to participate in the study by phone call or personal contact by the research team fieldworkers.

Participants of this cohort study. Pregnant women over 15 years of age who presented with a macular
or papular rash and two or more other symptoms, including fever, arthralgia, headache, myalgia, or non-purulent conjunctivitis, in accordance to definition of suspected ZIKV cases adopted by the Brazilian Ministry of Health 43 . No exclusion criteria were applied. After signing the Free and Informed Consent Form, women answered a structured questionnaire regarding sociodemographic, clinical-epidemiological, and reproductive characteristics. We used standardised protocols and forms developed by the Microcephaly Epidemic Research Group (MERG) from Pernambuco, Brazil, to collect data 24 . We investigated current and past medical and obstetric conditions. The pregnant women were asked about the characteristics of the rash and associated signs and symptoms, in addition to potential maternal comorbidities that could affect fetal growth and/or development. The interviews were conducted by trained health professionals from our research team.

Laboratory tests.
As part of the public health surveillance during the study period, pregnant women with suspected ZIKV infections who attended public health units in the Goiânia metropolitan region were invited to provide blood and/or urine samples for confirmatory tests, which were performed at the Central Laboratory of Public Health Dr. Giovanni Cysneiros in Goiás State (LACEN-GO). Molecular testing with reverse transcription-polymerase chain reaction (RT-PCR) was performed within the first five days of symptom onset for blood samples and up to 10 days after symptom onset for urine samples. Serology was performed for blood samples collected after the 5th day of symptom onset to detect immunoglobulin (Ig)M antibodies to ZIKV and DENV 51 .
At baseline interview, pregnant women provided blood and urine samples for additional molecular (ZIKV and chikungunya virus-CHIKV) and serological tests (ZIKV, DENV, and CHIKV). Samples were sent to the Laboratory of Virology and Cell Culture (LabVICC) of the Institute of Tropical Pathology and Public Health, and to the Laboratory of Molecular Biology (BioTec) of the Pharmacy Faculty of the Universidade Federal de Goiás (UFG). For infants with brain abnormalities with or without microcephaly, we collected blood and/or urine samples for RT-PCR ZIKV detection of ZIKV.
For the RT-PCR assays, the viral RNA was extracted from 140 µL of serum samples using the QIAamp® Viral RNA Mini Kit (Qiagen, Germany), following the manufacturer's instructions. The RT-PCR assays used probes and primers previously described for ZIKV 52  Pregnancy follow-up. Pregnant women were followed-up every four to eight weeks until delivery by the project team, independent of their routine prenatal care. All follow-up visits (consultations) were carried out with the assistance of a single obstetrician (LEPR) for consistency and quality assurance. Spontaneous fetal loss before the 22nd week of pregnancy was defined as miscarriage. A fetal loss after this period was defined as stillbirth. The birth of a live newborn before the 37th week of pregnancy was considered a premature birth.
Pediatric follow-up and definitions. Newborn data, including anthropometric measurements, morphological and functional status were extracted from medical records. Children were referred to specialized health care units (Hospital Materno Infantil, Hospital das Clinicas and Centro de Referencia em Oftalmologia, CEROF da UFG). They were evaluated by neonatologists, pediatricians, pediatric neurologists and pediatric ophthalmologists, of the research team using standardised forms and protocols. Pediatric visits were scheduled as early as possible in the neonatal period and then at 3, 6, and 12 months of life. Children were monitored as clinically indicated with additional medical visits. www.nature.com/scientificreports/ Newborns weighing less than 2500g at birth were considered low birth weight, and small-for-gestationalage (SGA) was defined as a birth weight less than the 10th percentile for gestational age and sex based on the INTERGROWTH-21st curves 56,57 .
Microcephaly was defined as head circumference (HC) (i.e., measured at birth and confirmed by physical examination between 24 and 48 hours afterward) below two standard deviations for gestational age and sex of the reference values of the INTERGROWTH-21st curves for boys and girls. Disproportionate microcephaly was defined as a HC z-score of < −2 with a normal birth weight score of > −2 56 . Postnatal-onset microcephaly was defined for children with no evidence of microcephaly at birth but who had HC measurements collected >2 weeks after birth, that fell below the 3rd percentile of the curve for the child's sex and age based on the World Health Organization child growth standards 58 .
Ophthalmological evaluation was performed by two ophthalmologists (at least one of them a retinal specialist) and image results were reviewed by two other specialists blinded to the mothers' ZIKV infection status. Ophthalmoscopy was performed with a Riester All Pupil II-Xenon indirect binocular ophthalmoscope (Rudolf Riester GmbH, Germany) and a non-contact lens (Double Aspherics) of 28 diopters (Volk, United States). Photo documentation was performed with a Topcon TRC-50IX Retinal Camera® (Topcon Medical Systems) (TOPCON CORPORATION, 2008) or Visucam Fundus Imaging 524® (Carl Zeiss Meditec Group) (ZEISS, 2017).
For children with clinically presumed heart defects transthoracic echocardiograms (ECHO) with standard pediatric views were performed at the neonatal period. Minor ECHO abnormalities were defined as persistent foramen ovale (PFO), small physiological tricuspid regurgitation, physiological pulmonary stenosis and patent ductus arteriosus in premature infants. Other heart defects were classified as major. Severe birth defects were defined as the ones requiring therapeutic interventions in the first days of life 39,59 .
We considered microcephaly, brain imaging abnormalities, neurological and/or ophthalmologic abnormalities as signs and symptoms potentially associated with CZS according to the Brazilian guidelines 43 . Congenital Zika Syndrome with severity categorized based on a combination of major and minor signs (neuroimaging exams, clinical signs and ocular anomalies) proposed by Pomar and colleagues 15,44 We considered as neurological or neurodevelopmental abnormalities: seizures, developmental delay, cognitive disabilities, localized motor deficit, hypertonia, alterations in tone or trophism, signs of pyramidal release, swallowing disorders, and movement disorders 16 .
Pre-and postnatal ultrasound exams. To determine the gestational age and subsequently to identify fetal anomalies, at least two ultrasounds were performed, one at the time of recruitment and one in the third trimester of pregnancy, between the 32nd and 37th gestational weeks. For the confirmation of gestational age, a first ultrasound performed in the first 12 weeks of gestation was preferred, which was compared to the date of the last menstruation provided by the pregnant woman. Whenever possible, fetal morphological ultrasound was also requested between 22 and 26 weeks of gestation 60 .
Antenatal ultrasound exams done at the referral hospital (Hospital Materno Infantil) were performed by a single examiner (fetal medicine specialist), with a 3.5 MHz convex transducer (Philips Healthcare device, model HD7), to measure the fetal head circumference (HC), other biometric parameters and estimate fetal weight. The diagnosis of intrauterine microcephaly was performed when the HC measurement was less than 2 standard deviations from the lower limit of the normality curve for the respective gestational age 61,62 .
Transfontanellar ultrasound was performed at the availability at the maternity hospital where the delivery took place and later on until the first six months of life, to investigate Central Nervous System abnormalities. Qualified physicians performed imaging examination, following a protocol for evaluating and describing results.
Fetuses and infants outcomes. Primary outcomes included: (i) microcephaly within one year of age and (ii) Congenital Zika Syndrome. The secondary outcomes were: (i) fetal loss, (ii) prenatal microcephaly detected by ultrasound, (iii) microcephaly at birth, and (iv) ophthalmological anomalies (retinal and/or optic nerve).
Data processing and statistical analysis. All data collected were stored using Research Electronic Data Capture (REDCap). After assessing the consistency of the data, statistical analyses were performed using IBM SPSS Statistics 25 version. For continuous variables with normal distribution, we used the student's t-test. Otherwise, the Mann-Whitney U test was used to compare these variables. We applied the Chi-squared test or Fisher's exact tests for categorical variables. p values of less than 0.05 were considered statistically significant. We estimated the absolute risk and 95% confidence interval (95% CI) for primary and secondary adverse outcomes among ZIKV-exposed children.

Data availability
The datasets generated during and analysed during the current study are available from the corresponding author on reasonable request. www.nature.com/scientificreports/